A gene used to identify which bacteria have taken up a recombinant plasmid (general term)

Placing a DNA fragment into a vector

Short overhanging single stranded sections of DNA left after it has been cut by a restriction endonuclease

A sequence of DNA where RNA polymerase and transcription factors will bind to begin the process of transcription

Enzyme that converts mRNA to cDNA

The DNA of two different organisms that has been combined

Something which can transfer DNA into a cell e.g. a plasmid or a virus

Copying DNA in the lab using PCR

Machine used in PCR to vary temperatures

The gene which produces this enzyme can be used as a marker, as the enzyme will turn a colourless substrate blue

In PCR, short sequences of nucleotides which are complementary to the start and end of the sequence to be copied

Used to increase permeability of bacterial membrane and therefore increase the chance of transformation

Joins the sugar phosphate backbone to attach pieces of DNA together

A sequence of DNA which releases RNA polymerase and ends transcription

Enzymes which cut DNA at a specific base sequence

The palindromic base sequence which is cut by a restriction endonuclease

Using a vector to put DNA into a host cell which will then copy the DNA when it divides